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João H. C. Manfrão-Netto Enzo Bento Queiroz Ana C. de Oliveira Junqueira Antônio M. V. Gomes Daniel Gusmão de Morais Hugo Costa Paes Nádia Skorupa Parachin 《Journal of applied microbiology》2022,132(2):822-840
Hyaluronic acid (HA) is a biopolymer of repeating units of glucuronic acid and N-acetylglucosamine. Its market was valued at USD 8.9 billion in 2019. Traditionally, HA has been obtained from rooster comb-like animal tissues and fermentative cultures of attenuated pathogenic streptococci. Various attempts have been made to engineer a safe micro-organism for HA synthesis; however, the HA titres obtained from these attempts are in general still lower than those achieved by natural, pathogenic producers. In this scenario, ways to increase HA molecule length and titres in already constructed strains are gaining attention in the last years, but no recent publication has reviewed the main genetic strategies applied to improve HA production on heterologous hosts. In light of that, we hereby compile the advances made in the engineering of micro-organisms to improve HA synthesis. 相似文献
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Pimenta Larissa Langsdorff Lima Gustavo Pereira Biondi Michel Vaz Marcelo Gomes Marçal Vieira de Freitas Coelho Flávia 《Aquatic Ecology》2022,56(3):543-553
Aquatic Ecology - In epiphytic associations, cyanobacteria form the periphyton with phytoplanktonic algae and with aquatic macrophytes. In this study, we found homocytous and heterocytous... 相似文献
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Maria I. B. de Oliveira Lorena V. de Matos Lídia A. da Silva Edsandra C. Chagas Grazyelle S. da Silva Ana L. S. Gomes 《Journal of fish biology》2019,94(4):648-659
The objective of the present study was to describe the histology and histochemistry of the mucosal layer of the digestive tube of Piaractus brachypomus, and the histopathology associated with parasitism by Neoechinorhynchus sp. The digestive tube of P. brachypomus consists of three macroscopically distinct portions: short, rectilinear and elastic-walled ooesophagus, J-shaped siphon stomach and a long intestine with rectilinear and curved portions, defined by patterns of villi as foregut, midgut, and hindgut. Histological and histochemical differences were observed in the mucosal layers of the different digestive tube regions, such as intense production of neutral and acidic mucous substances in the pseudostratified mucosal epithelium of the oesophagus; positive periodic acid Schiff reagent (PAS)reactions at the apex of the columnar epithelial cells of the stomach and increased intensity of histochemical reactions in the hindgut region. Neoechinorhynchus sp. was present in 85.7% of specimens examined, with a mean intensity of 7.4 ± 6.2 (±) and abundance of 6.33. Good health of the fish indicated by high relative condition factor values ( Kn) and occurrence of only mild to moderate alteration in the mucosal layer indicated that Neoechinorhynchus sp. exhibits low pathogenicity towards P. brachypomus hosts in farming environments, with low levels of infection. 相似文献
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Alirya Magda Santos do Vale Gomes Fabrício de Oliveira Reis Raimunda Nonata Santos de Lemos Janaína Marques Mondego Heder Braun José Ribamar Gusmão Araujo 《Revista Brasileira de Entomologia》2019,63(2):119-123
Aleurocanthus woglumi (Ashby, 1915) is an important agricultural pest that causes yield losses of 20–80% in citrus plants by removing plant nutrients while feeding and allowing the formation of sooty mold. The objective of this study was to evaluate physiological changes in citrus plants in response to A. woglumi infestation under field conditions. The experiment was conducted in a citrus orchard in Paço do Lumiar, Maranhão, Brazil. Thirty-two citrus plants were used, including eight of each of the following varieties: Tahiti lime, Tanjaroa tangerine, Nissey tangerine, and Ponkan tangerine. Four random plants with A. woglumi infestation and four plants free from this pest were selected from each variety. The physiological parameters evaluated were photochemical efficiency and gas exchange. Regarding photochemical efficiency, infested plants presented photoinhibition damage, with a performance index of 4.22. The gas exchange parameters of infested plants changed, with reductions in photosynthetic CO2 assimilation of 69.7% (Tahiti), 64% (Tanjaroa), 68.8% (Nissey) and 63.3% (Ponkan). Plants infested with A. woglumi also presented physiological changes; their photosynthetic CO2 assimilation, stomatal conductance, instantaneous transpiration, and performance indexes were affected. The infested citrus plants showed photoinhibition of photosystem II. The photosynthetic CO2 assimilation decreased approximately 70% in Tahiti lime, Tanjaroa tangerine, Nissey tangerine, and Ponkan tangerine plants infested with A. woglumi. 相似文献
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Freitas Natália Chagas Barreto Horllys Gomes Torres Luana Ferreira Freire Luciana Lima Rodrigues Leonardo Augusto Zebral Diniz Leandro Eugenio Cardamone Beijo Luiz Alberto Paiva Luciano Vilela 《Plant Cell, Tissue and Organ Culture》2019,137(3):599-611
Plant Cell, Tissue and Organ Culture (PCTOC) - The employment of biotechnology-based approaches such as somatic embryogenesis has been applied to several plants including Coffea sp. Despite the... 相似文献